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KMID : 0811720080120010001
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Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 1 p.1 ~ p.6
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Effects of TGF-β1 Ribbon Antisense on CCl4-induced Liver Fibrosis
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Doh Kyung-Oh
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Abstract
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Ribbon-type antisense oligonucleotide to TGF-β1 (TGF-β1 RiAS) was designed and tested to prevent or resolve the fibrotic changes induced by CCl4 injection. When Hepa1c1c7 cells were transfected with TGF-β1 RiAS, the level of TGF-β1 mRNA was effectively reduced. TGF-β1 RiAS, mismatched RiAS, and normal saline were each injected to mice via tail veins. When examined for the biochemical effects on the liver, TGF-β1 mRNA levels were significantly reduced only in the TGF-β1 RiAS-treated group. The results of immunohistochemical studies showed that TGF-β1 RiAS prevented the accumulation
of collagen and α-smooth muscle actin, but could not resolve established fibrosis. These results indicate that ribbon antisense to TGF-β1 with efficient uptake can effectively prevent fibrosis of the liver.
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KEYWORD
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Transforming growth factor-β1, Liver cirrhosis, Ribbon antisense, Cationic peptide
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